STEC non-0157 strains in meat from Southern Sonora, Mexico and its antibiotic resistance

Escherichia coli is one of the most important bacteria associated with consumption meat products causing human disease, bovines as main reservoir for this organism. Microbiological quality raw sampled at slaughterhouses was evaluated by measuring presence serogroups that produce Shiga toxin (referred to STEC), including both O157:H7 and non-O157 strains can cause Hemolytic Uremic Syndrome (HUS). The purpose study detect STEC O157 in from Federal Inspection Type (FIT, n=52) non-FIT (n=88) slaughterhouses, well butcheries (n=50), southern Sonora, Mexico. To achieve aim, selective media were employed obtain putative colonies (TS-SMAC agar Rainbow® O157), followed multiplex PCR stx1, stx2, eaeA, rfB genes, using serotype a control. No found beef products, but some isolates containing stx2 pathogenicity gene (1.40%, 3/224) and/or fliCH4 flagellar antigen (1.78%, 4/224). This work also confirmed susceptibility genes several antibiotics multi-resistance all or these trimethoprim/sulfamethoxazole, tetracycline, clarithromycin, penicillin G, oxacillin, ampicillin cefazolin. comprises first report Sonora will be useful establish their association foodborne outbreaks originating pork future.